Virus:Article Title: On-Demand Patient-Specific Phenotype-to-Genotype Ebola Virus Characterization
Article Snippet: .. Cell lines used for virus rescues included human cervix epithelial HeLa cells (American Type Culture Collection [ATCC], Manassas, VA, USA; ATCC CRM-CCL-2), HEK 293T cells (ATCC CRL-11268); human hepatocellular carcinoma epithelial Huh7 cells (Health Science Research Resources Bank [JCRB0403], Osaka, Japan), Huh7D-12/T7 (a Huh7 cell line stably expressing hepatitis delta antigen [HDAg] from Millipore Sigma, St. Louis, MO, USA, modified by Genecopoeia Rockville, MD, USA to stably express T7 RNA polymerase for this study), and grivet kidney epithelial Vero C1008/E6 cells (BEI Resources, Manassas, VA, USA; Catalog Number [Cat. No.] NR596). .. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) +10% heat-inactivated fetal bovine serum (FBS; GE Healthcare Life Sciences, Pittsburgh, PA, USA and Sigma) at 37 °C and 5% carbon dioxide (CO 2 ).
Stable Transfection:Article Title: On-Demand Patient-Specific Phenotype-to-Genotype Ebola Virus Characterization
Article Snippet: .. Cell lines used for virus rescues included human cervix epithelial HeLa cells (American Type Culture Collection [ATCC], Manassas, VA, USA; ATCC CRM-CCL-2), HEK 293T cells (ATCC CRL-11268); human hepatocellular carcinoma epithelial Huh7 cells (Health Science Research Resources Bank [JCRB0403], Osaka, Japan), Huh7D-12/T7 (a Huh7 cell line stably expressing hepatitis delta antigen [HDAg] from Millipore Sigma, St. Louis, MO, USA, modified by Genecopoeia Rockville, MD, USA to stably express T7 RNA polymerase for this study), and grivet kidney epithelial Vero C1008/E6 cells (BEI Resources, Manassas, VA, USA; Catalog Number [Cat. No.] NR596). .. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) +10% heat-inactivated fetal bovine serum (FBS; GE Healthcare Life Sciences, Pittsburgh, PA, USA and Sigma) at 37 °C and 5% carbon dioxide (CO 2 ).
Expressing:Article Title: On-Demand Patient-Specific Phenotype-to-Genotype Ebola Virus Characterization
Article Snippet: .. Cell lines used for virus rescues included human cervix epithelial HeLa cells (American Type Culture Collection [ATCC], Manassas, VA, USA; ATCC CRM-CCL-2), HEK 293T cells (ATCC CRL-11268); human hepatocellular carcinoma epithelial Huh7 cells (Health Science Research Resources Bank [JCRB0403], Osaka, Japan), Huh7D-12/T7 (a Huh7 cell line stably expressing hepatitis delta antigen [HDAg] from Millipore Sigma, St. Louis, MO, USA, modified by Genecopoeia Rockville, MD, USA to stably express T7 RNA polymerase for this study), and grivet kidney epithelial Vero C1008/E6 cells (BEI Resources, Manassas, VA, USA; Catalog Number [Cat. No.] NR596). .. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) +10% heat-inactivated fetal bovine serum (FBS; GE Healthcare Life Sciences, Pittsburgh, PA, USA and Sigma) at 37 °C and 5% carbon dioxide (CO 2 ).
Modification:Article Title: On-Demand Patient-Specific Phenotype-to-Genotype Ebola Virus Characterization
Article Snippet: .. Cell lines used for virus rescues included human cervix epithelial HeLa cells (American Type Culture Collection [ATCC], Manassas, VA, USA; ATCC CRM-CCL-2), HEK 293T cells (ATCC CRL-11268); human hepatocellular carcinoma epithelial Huh7 cells (Health Science Research Resources Bank [JCRB0403], Osaka, Japan), Huh7D-12/T7 (a Huh7 cell line stably expressing hepatitis delta antigen [HDAg] from Millipore Sigma, St. Louis, MO, USA, modified by Genecopoeia Rockville, MD, USA to stably express T7 RNA polymerase for this study), and grivet kidney epithelial Vero C1008/E6 cells (BEI Resources, Manassas, VA, USA; Catalog Number [Cat. No.] NR596). .. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) +10% heat-inactivated fetal bovine serum (FBS; GE Healthcare Life Sciences, Pittsburgh, PA, USA and Sigma) at 37 °C and 5% carbon dioxide (CO 2 ).
Article Title: Cyclophilin A stabilizes the capsid protein p72 to facilitate African swine fever virus replication.
Article Snippet: Primary porcine alveolar macrophages (PAMs) were isolated from 5-week-old healthy specific-pathogen-free (SPF) Landrace piglets, regardless of sex, as previously described79, 80, and were maintained in RPMI-1640 medium (Gibco, #C11875500BT) supplemented with 20% porcine serum (HyClone, #SH30908.04), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, #15140122), and 2 mmol/mL glutamine at 37 °C with 5% CO2 in a humidified incubator. .. The wild boar kidney cell line (BK2258), human embryonic kidney 293T (HEK293T, CRL-3216, ATCC), and human cervix epithelial HeLa cells (CCL-2, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, #C11995500BT) containing with 10% Fetal bovine serum (FBS; Sigma, #F8687-500mL) and 1% penicillin/streptomycin at 37 °C with 5% CO2. .. For transfection experiments, cells were transfected using Lipo293 (Beyotime, #C0521), lipofectamine 2000 (Life Technologies, #11668019), or polyethylenimine (PEI; Polysciences, #23966-100), according to the manufacturer's instructions.
Cell Culture:Article Title: Nanoscale Label-free Bioprobes to Detect Intracellular Proteins in Single Living Cells
Article Snippet: .. Human cervix epithelial HeLa cells (American Type Culture Collection, ATCC) were cultured at 37°C in DMEM F-12 Medium supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. Western blot (see ) was conducted to validate the affinity of the anti-p53 (Santa Cruz) to the commercial p53 protein (Santa Cruz) as well as to the p53 protein from the HeLa cell lysis.
Article Title: Systems and methods for making and using sensors, probes, and other devices
Article Snippet: .. Human cervix epithelial HeLa cells (American Type Culture Collection, ATCC) were cultured at 37° C. in DMEM F-12 Medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, and 100 mg/ml streptomycin. .. The cells were harvested, lysed, and the total protein concentration was measured (10 mg/mL) by a Bradford assay (Thermo Scintific).
Article Title: Cyclophilin A stabilizes the capsid protein p72 to facilitate African swine fever virus replication.
Article Snippet: Primary porcine alveolar macrophages (PAMs) were isolated from 5-week-old healthy specific-pathogen-free (SPF) Landrace piglets, regardless of sex, as previously described79, 80, and were maintained in RPMI-1640 medium (Gibco, #C11875500BT) supplemented with 20% porcine serum (HyClone, #SH30908.04), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, #15140122), and 2 mmol/mL glutamine at 37 °C with 5% CO2 in a humidified incubator. .. The wild boar kidney cell line (BK2258), human embryonic kidney 293T (HEK293T, CRL-3216, ATCC), and human cervix epithelial HeLa cells (CCL-2, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, #C11995500BT) containing with 10% Fetal bovine serum (FBS; Sigma, #F8687-500mL) and 1% penicillin/streptomycin at 37 °C with 5% CO2. .. For transfection experiments, cells were transfected using Lipo293 (Beyotime, #C0521), lipofectamine 2000 (Life Technologies, #11668019), or polyethylenimine (PEI; Polysciences, #23966-100), according to the manufacturer's instructions.
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